Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identifying mRNAs bound by RNA-binding proteins using affinity purification and differential display.

Nancy D Rodgers1, Xinfu Jiao, Megerditch Kiledjian

  • 1Department of Cell Biology and Neuroscience, Nelson Biology Laboratories, Rutgers University, 604 Allison Road, Piscataway, NJ 08854, USA.

Methods (San Diego, Calif.)
|June 11, 2002
PubMed
Summary

Identifying RNA-protein interactions is crucial for understanding genetic disorders. The new isolation of specific nucleic acids associated with proteins (SNAAP) technique helps discover unknown RNA targets of RNA-binding proteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular exosomal RNAs are glyco-modified.

Nature cell biology·2025
Same author

Analysis of ligand binding mechanism by dimeric receptors using stopped-flow fluorimetry-application to the human decapping scavenger enzyme.

European biophysics journal : EBJ·2025
Same author

Biallelic NUDT2 variants defective in mRNA decapping cause a neurodevelopmental disease.

Brain : a journal of neurology·2023
Same author

NADcapPro and circNC: methods for accurate profiling of NAD and non-canonical RNA caps in eukaryotes.

Communications biology·2023
Same author

Preparation of RNAs with non-canonical 5' ends using novel di- and trinucleotide reagents for co-transcriptional capping.

Frontiers in molecular biosciences·2022
Same author

Identification of a novel deFADding activity in human, yeast and bacterial 5' to 3' exoribonucleases.

Nucleic acids research·2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Numerous methods exist to identify specific RNA-protein interactions.
  • However, identifying all RNA targets of a given RNA-binding protein remains challenging.
  • This is critical as many RNA-binding proteins are implicated in genetic disorders.

Purpose of the Study:

  • To present a novel technique, isolation of specific nucleic acids associated with proteins (SNAAP), for identifying unknown mRNA substrates of RNA-binding proteins.
  • To detail the methodology for expressing and purifying fusion proteins for substrate RNA isolation.
  • To describe experimental validation of identified RNA-protein interactions.

Main Methods:

  • Isolation of Specific Nucleic Acids Associated with Proteins (SNAAP) technique.

Related Experiment Videos

  • Expression and purification of fusion proteins.
  • Differential display technology for RNA isolation.
  • Experimental validation of RNA-protein interactions.

    • Main Results:

    • The SNAAP technique enables the identification of specific mRNAs bound by a protein of interest.
    • Detailed protocols are provided for implementing the SNAAP method.
    • Validation experiments confirm the identified RNAs as bona fide substrates.

    Conclusions:

    • The SNAAP technique provides a valuable tool for discovering novel RNA targets of RNA-binding proteins.
    • This methodology is essential for advancing the understanding of RNA-protein interactions in health and disease.
    • Improved identification of RNA-protein interactions can lead to better insights into genetic disorder mechanisms.