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Updated: Aug 4, 2026
08:17
Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major
Published on: October 28, 2022
A kinetoplast DNA minicircle probe (B18) specific for the Leishmania subgenus Viannia
L J Gibson1, S H E McCann, S Brewster
1Imperial College School of Medicine, Charing Cross Hospital, London, UK.
Abstract:
This paper reports the development, sequence and specificity of probe B18, which hybridizes only to the kinetoplast deoxyribonucleic acid (kDNA) minicircle of Leishmania species of the subgenus Viannia. This probe was developed in 1985 and has been used extensively since, on whole kDNA, Leishmania dot-blots and kDNA minicircles amplified by the polymerase chain reaction.
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