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Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
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The influence of storage time upon the real thickness of the histological brain sections

J Dziewiatkowski1, J H Spodnik, S Wójcik

  • 1Department of Anatomy and Neurobiology, Medical University, Gdańsk, Poland. jdziew@amg.gda.pl

Folia Histochemica Et Cytobiologica
|June 12, 2002
PubMed

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Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...