Development of mucous cells in taste discs of anurans

Differentiation of the lingual taste discs (TDs) was studied in tadpoles of Rana esculenta, Hyla arborea and Pelobatesfuscus by means of LM, TEM, and SEM. The process of differentiation of mucous cells (MCs) within a TD anlage was investigated subsequently from 39th to 46th Gosner's developmental stage. In R. esculenta and P. fuscus, the MCs are distinguishable in TD anlages not earlier than at the 42nd tadpole Gosner's stage. At that stage TDs anlages are covered by a single layer of ordinary epithelial cells. Starting from the stage 44, tips of TDs progressively open outside and free surfaces of MCs can be distinguished as an area with numerous microfolds. At developmental stages 45 and 46, numerous electron-dense secretory granules can be seen in the supranuclear cytoplasm of MCs. The presence of mucopolysaccharides in H. arborea and P. fuscus was observed from the 44th-45th tadpole developmental stage.