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Phage lambda red-mediated adaptive mutation.

Anthony R Poteete1, Hsinju R Wang, Patricia L Foster

  • 1Department of Molecular Genetics & Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA. anthony.poteete@umassmed.edu

Journal of Bacteriology
|June 12, 2002
PubMed
Summary
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Adaptive mutation, a process where cells adapt to environmental stress, occurs more frequently in E. coli strains using bacteriophage lambda

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Adaptive mutation allows cells to adapt to environmental stress.
  • The recBCD genes in E. coli are involved in DNA repair and recombination.
  • The red recombination genes from bacteriophage lambda are known to mediate homologous recombination.

Purpose of the Study:

  • To investigate the role of bacteriophage lambda's red recombination genes in adaptive mutation in Escherichia coli.
  • To compare Red-mediated adaptive mutation with RecBCD-dependent adaptive mutation.

Main Methods:

  • Replacing the recBCD genes in E. coli with the red recombination genes.
  • Analyzing the frequency of adaptive mutation in the modified E. coli strain.
  • Investigating the dependence of Red-mediated adaptive mutation on recA and ruvABC genes.

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Main Results:

  • Adaptive mutation occurred at an elevated frequency in E. coli strains where recBCD genes were replaced by lambda's red genes.
  • Red-mediated adaptive mutation was found to be dependent on recA and ruvABC functions, similar to RecBCD-dependent adaptive mutation.

Conclusions:

  • The red recombination genes of bacteriophage lambda can substitute for recBCD in promoting adaptive mutation in E. coli.
  • Both RecBCD-dependent and Red-mediated adaptive mutation pathways require recA and ruvABC functions, suggesting a conserved mechanism for adaptive mutation in E. coli.