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Cod1p/Spf1p is a P-type ATPase involved in ER function and Ca2+ homeostasis
Stephen R Cronin1, Rajini Rao, Randolph Y Hampton
1Division of Biology, University of California San Diego, La Jolla, CA 92093-0347, USA.
The Journal of Cell Biology
|June 12, 2002
Summary
Cod1p, a novel P-type ATPase, regulates endoplasmic reticulum (ER) function and calcium homeostasis. This study biochemically characterizes Cod1p, revealing its conserved role in ion transport and ER health.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- The endoplasmic reticulum (ER) internal environment is crucial for cellular processes.
- Understanding ER regulation is incomplete.
- Cod1p/Spf1p is an uncharacterized type V P-type ATPase potentially involved in ER function.
Purpose of the Study:
- To biochemically characterize Cod1p, a type V P-type ATPase.
- To investigate Cod1p's role in ER function and ion homeostasis.
- To determine if Cod1p's functions are conserved in metazoans.
Main Methods:
- Localization of Cod1p to the ER membrane.
- Analysis of cod1Delta mutant phenotypes (calcium homeostasis, gene transcription, ER stress response).
- Complementation studies with Drosophila melanogaster homologue and biochemical assays of purified protein.
Main Results:
- Cod1p localizes to the ER membrane.
- cod1Delta mutants exhibit disrupted calcium homeostasis and ER dysfunction (UPR activation, tunicamycin sensitivity).
- Cod1p ATPase activity was demonstrated, and its function is conserved in Drosophila.
Conclusions:
- Cod1p is a type V P-type ATPase involved in ER ion homeostasis.
- Cod1p plays a critical role in maintaining ER function.
- Cod1p's role in ion transport and ER homeostasis is conserved across metazoans.