Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microtubule Formation

Microtubule Formation

Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation of...

Septins

Septins

Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...

Coat Assembly and GTPases

Coat Assembly and GTPases

Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...

The Contractile Ring

The Contractile Ring

Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...

Role of Septins

Role of Septins

Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...

The Contractile Ring

The Contractile Ring

Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Common Collagen of Alport Syndrome and Arthritis: A Case Report and Review of Pathophysiology.

Case reports in nephrology·2026

Same author

Leveraging nonlinear relationships and interactions to improve 30-day pneumonia readmission machine learning models.

PloS one·2026

Same author

Novel tool use does not depend on mechanical reasoning: evidence from apraxia.

bioRxiv : the preprint server for biology·2026

Same author

Prediabetes and Obstructive Sleep Apnea: A review of the pathophysiological interaction and the role of CPAP.

Sleep medicine·2026

Same author

From acute infection to chronic risk: reframing pneumonia outcomes through long-term mortality prediction.

American journal of respiratory and critical care medicine·2026

Same author

A MAP kinase cascade modulates expression of late G1 phase cyclins in budding yeast.

Molecular biology of the cell·2026

Search research articles

Related Experiment Video

Updated: Jul 25, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Cell cycle-dependent assembly of a Gin4-septin complex.

Eric M Mortensen¹, Hayes McDonald, John Yates

¹Department of Molecular, Cellular, and Developmental Biology, Sinsheimer Labs, University of California, Santa Cruz, California 95065, USA.

Molecular Biology of the Cell

|June 12, 2002

Summary

Gin4 kinase activity is activated during mitosis through self-phosphorylation within a protein complex. This complex, involving Shs1 septin, targets Shs1 for phosphorylation, regulating cell growth and septin localization.

More Related Videos

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Published on: June 23, 2022

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Published on: July 28, 2022

Related Experiment Videos

Last Updated: Jul 25, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Published on: June 23, 2022

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Published on: July 28, 2022

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Gin4 is a kinase regulating cell division and septin localization in budding yeast.
Gin4 activation is linked to its hyperphosphorylation during mitosis.

Purpose of the Study:

Identify proteins interacting with Gin4 during mitosis.
Discover Gin4 kinase targets and regulators of its activation.

Main Methods:

Immunoaffinity chromatography to isolate Gin4-binding proteins.
In vitro kinase assays.
Genetic analysis.

Main Results:

Gin4 forms a complex with Nap1, Bni5, septins (including Shs1), and other Gin4 molecules during mitosis.
Gin4 molecules within the complex phosphorylate each other, leading to hyperphosphorylation and activation.
Shs1 is a Gin4 substrate, and its interaction with Gin4 is crucial for Gin4 complex formation and self-association.
Gin4-septin association requires Shs1, Nap1, Cla4, Elm1, Gin4, and Cdc28 kinase activities.

Conclusions:

Gin4 activation is tightly regulated by protein-binding events, particularly its association with Shs1 during mitosis.
Shs1 acts as a key regulator and likely substrate for Gin4, influencing its kinase activity and localization.
These findings provide insights into how Gin4 regulates septin function and localization during cell division.