Confocal Raman and fluorescence spectroscopy applied to polymeric chromatographic adsorbent particles
Related Experiment Video
Updated: Sep 30, 2026

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
Published on: April 12, 2017
Mina Larsson1, Jan Lindgre, Anders Ljunglöf
1Uppsala University, The Angström Laboratory, Materials Chemistry, Sweden.
Abstract:
Distributions of functional groups in individual chromatographic adsorbent particles have been investigated using confocal Raman and fluorescence spectroscopy. Measurements have been performed with different microscope objectives and it was found that an immersion objective should be used when wet adsorbent particles are analysed. Nd3+ ions have been used as fluorescence probes to evaluate the distribution of chelating and negatively charged functional groups. The fluorescence spectrum from Nd3+ has also been used to obtain information about the coordination of Nd3+ within the adsorbent particles.
Related Concept Videos
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Analyte Adsorption and Distribution
Affinity Chromatography

