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Updated: Sep 30, 2026

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
Published on: May 13, 2022
Liquid exclusion-adsorption chromatography: a new technique for isocratic separation of non-ionic surfactants. V.
Bernd Trathnig1, Christina Rappel, Reingard Raml
1Institute of Chemistry, Karl-Franzens-University Graz, Austria. trathnig@kfunigraz.ac.at
Abstract:
Fatty acid polyglycol esters can be fully characterized using two-dimensional liquid chromatography with liquid chromatography under critical conditions (LCCC) as the first and liquid exclusion-adsorption chromatography (LEAC) as the second dimension. LEAC is run under isocratic conditions, which allows the use of the refractive index detector, and thus accurate quantitation. Fractions from LCCC are transferred to LEAC using the full adsorption-desorption technique, by which they are focussed and reconcentrated before injection into the second dimension. This is achieved by increasing the water content of the mobile phase behind the LCCC column. Monoester oligomers of up to 20 oxyethylene units can be resolved to the baseline. Diester oligomers are partially separated in the first dimension (LCCC).
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