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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Published on: April 19, 2014
Golgi secretion is not required for marking the preprophase band site in cultured tobacco cells
1The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA. rvd10@psu.edu
Abstract:
The preprophase band predicts the future cell division site. However, the mechanism of how a transient preprophase band fulfils this function is unknown. We have investigated the possibility that Golgi secretion might be involved in marking the preprophase band site. Observations on living BY-2 cells labeled for microtubules and Golgi stacks indicated an increased Golgi stack frequency at the preprophase band site. However, inhibition of Golgi secretion by brefeldin A during preprophase band formation did not prevent accurate phragmoplast fusion, and subsequent cell plate formation, at the preprophase band site. The results show that Golgi secretion does not mark the preprophase band site and thus does not play an active role in determination of the cell division site.
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