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Effects of recombinant staphylokinase on coronary thrombosis in Chinese experimental miniature swine
Jian-Xun Liu1, Xiao-Hong Shang, Jian-Hua Fu
1Department of Basic Medicine Science, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China. jianxun@ht.rol.cn.net
Aim:
To study the effects of recombinant staphylokinase (r-Sak) on coronary thrombosis, cardiac ischemia, and myocardial infarction in Chinese experimental miniature swine.
Methods:
Endarterium was injuried and coronary thrombi were formed gradually through direct electrical stimulation on the coronary artery of Chinese experimental miniature swine. Effects of r-Sak in vitro were studied through coronary pathological section, microkinematography, multi media graphic analysis, epicardial electrogram mapping, myocardium histo chemical stain, serum creatine phosphokinase (CPK), and hemorheology.
Results:
r-Sak showed a remarkable effect on coronary thrombus. Compared w ith the control group, the two dosage groups, r-Sak 0.45 mg/kg and 1.35 mg/kg, reduced the transverse section area of coronary thrombus (P<0.01), lessened the degree and range of cardiac ischemia (P<0.01), decreased the myocardial infarction area (P<0.01), the activity of CPK and blood viscosity (P<0.05), restrained platelet adhesion and aggregation, and reduced fibrinogen concentration (P<0.05).
Conclusion:
r-Sak could dissolve coronary thrombus obviously and lessen the pathologic reaction of myocardium.
