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Genotype of CYP3AP1 associated with CYP3A activity in Chinese Han population
Bing Zhu1, Guo-Lin Chen, Xiao-Ping Chen
1Pharmacogenetics Research Institute, Xiang-Ya School of Medicine, Central South University, Changsha 410078, China.
Aim:
To investigate the distribution of genotype of CYP3AP1 in Chinese Han population and the correlation with CYP3A activity.
Methods:
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed in CYP3AP1 genotype analysis; using midazolam as probe drug, CYP3A activity of 191 Chinese healthy subjects was measured by plasma 1'-hydroxymidazolam/midazolam (1'-OH-MDZ/MDZ) ratio at 1 h after oral administration of 7.5 mg midazolam.
Results:
There was significant difference of CYP3A activity in different genotypes of CYP3AP1 in vivo (P <0.05). The activity of CYP3A in homozygous A(-44) (CYP3AP1*3/CYP3AP1*3) is lower than heterozygous A(-44)G (CYP3AP1*1/CYP3AP1*3), and the CYP3A activity in homozygous G(-44) (CYP3AP1*1/CYP3AP1*1) is t he highest.
Conclusion:
There was association between the genotype of CYP3AP1 and increased activity of CYP3A in vivo.