Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA fragmentation and morphological changes in apoptotic human lymphocytes.

Stefan Czene1, Elisabetta Testa, Jonas Nygren

  • 1Department of Genetic and Cellular Toxicology, Stockholm University, Sweden. stefan.czene@genetics.su.se

Biochemical and Biophysical Research Communications
|June 14, 2002
PubMed
Summary

This study reveals distinct DNA fragmentation stages during apoptosis in human lymphocytes. Early fragmentation occurs in normal-density cells, followed by shrinkage and density increase with smaller DNA fragments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to the BfS Statement: Scientific Clarifications on Chromosomal Damage and RF-EMF Exposure.

Ecotoxicology and environmental safety·2026
Same author

Screening for anal high-grade squamous intraepithelial lesions with flexible endoscopy in high-risk individuals.

Scandinavian journal of gastroenterology·2026
Same author

On exposure-response interpretation and evidence synthesis in low-intensity RF-EMF research.

Environment international·2026
Same author

Ligation of Intersphincteric Fistula Tract for Perianal Fistula: Long-term Results, Functional Outcomes, and Predictors of Failure.

Diseases of the colon and rectum·2025
Same author

Patients' Experiences of Diagnostic and Therapeutic High-Resolution Endoscopy in Treating Anal Squamous Intraepithelial Lesions: A Qualitative Study.

Diagnostics (Basel, Switzerland)·2025
Same author

Exposure of human lymphocytes to sweeping-frequency ELF MF.

International journal of radiation biology·2025

Area of Science:

  • Cell biology
  • Molecular genetics
  • Radiation biology

Background:

  • Apoptosis, or programmed cell death, is a crucial biological process.
  • Understanding DNA fragmentation dynamics during apoptosis is vital for cell fate determination.

Purpose of the Study:

  • To investigate the temporal and physical characteristics of DNA fragmentation during apoptosis in human lymphocytes.
  • To correlate DNA fragmentation patterns with morphological and density changes in apoptotic cells.

Main Methods:

  • Human peripheral blood lymphocytes were irradiated and separated on density gradients at various time points.
  • Comet assay and pulsed field gel electrophoresis were used to analyze DNA fragmentation.
  • Morphological changes (chromatin condensation, cell shrinkage) and cell density were assessed.

Related Experiment Videos

Main Results:

  • Distinct stages of DNA fragmentation were identified, correlating with morphological and density changes.
  • >/=50 kbp DNA fragmentation occurred in normal-density cells.
  • Fragmentation to <50 kbp fragments coincided with cell shrinkage and increased cell density.

Conclusions:

  • Apoptosis involves temporally separated DNA fragmentation stages.
  • These stages are linked to specific cellular changes, including density and morphology.
  • The temporal separation may contribute to chromosomal aberrations in abortive apoptosis.