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A method for measuring oxygen distributions in tissue using frequency domain phosphorometry
Sergei A Vinogradov1, Maria A Fernandez-Seara, Benjamin W Dupan
1Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. vinograd@mail.med.upenn.edu
Abstract:
Phosphorescence lifetime of phosphors dissolved in biological fluids is dependent on the local oxygen concentration. When phosphor is added to the blood in vivo there is a distribution of lifetimes due to the distribution of oxygen concentrations in the blood of the sampled tissue volume. The distribution of oxygen can be assessed non-invasively and in practically real-time using frequency domain time-resolved phosphorometry. Here we describe a multi-frequency system for measurements of oxygen distributions in tissue in vivo.