Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Decarboxylase activity study of Pasteurella multocida].

L Karaivanov

    Veterinarno-Meditsinski Nauki
    |January 1, 1979
    PubMed
    Summary

    Ornithine decarboxylase activity is a key characteristic for identifying Pasteurella multocida strains. This enzyme

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Morphology of Pasteurella multocida bacteriophages.

    Canadian journal of microbiology·1984
    Same author

    [Phosphatase activity of Pasteurella multocida strains isolated from poultry and mammals].

    Veterinarno-meditsinski nauki·1984
    Same author

    [In vitro action of zinc bacitracin to eliminate drug resistance in Escherichia coli transconjugates].

    Veterinarno-meditsinski nauki·1984
    Same author

    [Metabolism of free amino acid in strains of Pasteurella multocida and their division into biological types].

    Veterinarno-meditsinski nauki·1984
    Same author

    [Biochemical tests for identifying Pasteurella multocida].

    Veterinarno-meditsinski nauki·1984
    Same author

    [Passive hemagglutination test with capsular and somatic antigens of Pasteurella multocida strains isolated from birds].

    Veterinarno-meditsinski nauki·1983

    Area of Science:

    • Bacteriology
    • Microbial Taxonomy
    • Animal Pathology

    Background:

    • Pasteurella multocida is a significant bacterial pathogen affecting various animal species.
    • Accurate identification and classification of P. multocida strains are crucial for disease control and veterinary diagnostics.
    • Decarboxylase enzyme activity has been explored as a potential taxonomic marker in bacterial species.

    Purpose of the Study:

    • To investigate the ornithine, lysine, and arginine decarboxylase activity in a collection of P. multocida strains.
    • To evaluate the utility of ornithine decarboxylase activity as a taxonomic characteristic for P. multocida species identification.
    • To correlate decarboxylase activity with the pathogenicity of P. multocida strains in a mouse model.

    Main Methods:

    • Biochemical analysis of 218 P. multocida strains using Moller's method to determine decarboxylase activity.
    • Isolation of strains from diverse geographical locations and animal hosts, including birds, pigs, and rabbits.
    • Pathogenicity testing in white mice and assessment of enzyme activity in gamma-irradiated strains.

    Main Results:

    • A high prevalence of positive ornithine decarboxylase activity (211/218 strains) was observed in P. multocida.
    • Low arginine decarboxylase activity was detected in 12 strains, and low lysine decarboxylase activity in 14 strains.
    • All P. multocida strains pathogenic to white mice exhibited ornithine decarboxylase activity.

    Conclusions:

    • Ornithine decarboxylase activity is a reliable taxonomic marker for P. multocida identification, complementing cultural, biochemical, and phage lysis methods.
    • Loss of ornithine decarboxylase activity in P. multocida strains, even after gamma-irradiation, correlates with reduced pathogenicity.
    • Decarboxylase profiling offers valuable insights into the taxonomy and virulence of P. multocida.

    Related Experiment Videos