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Updated: Sep 30, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Published on: August 5, 2022
DNA hypomethylating agents 5-aza-2'-deoxycytidine and valproate increase neuronal 5-lipoxygenase mRNA
1Department of Psychiatry, The Psychiatric Institute, University of Illinois at Chicago, Chicago, IL 60612, USA. HManev@psych.uic.edu
Abstract:
DNA methylation regulates gene expression. Neuronal 5-lipoxygenase expression increases during aging and during the proliferation of immature rat cerebellar granule neurons. In these cultures, we investigated the effects of hypomethylating agents 5-aza-2'-deoxycytidine (AdC) and valproate on 5-lipoxygenase mRNA. Both drugs increased 5-lipoxygenase mRNA in proliferating cells; only valproate was effective in differentiated neurons. We propose that neuronal 5-lipoxygenase expression can be affected by aging-altered DNA methylation and by hypomethylating drugs, such as the anticonvulsant valproate.
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