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Chymase participates in chronic dermatitis by inducing eosinophil infiltration
Yoshiaki Tomimori1, Tsuyoshi Muto, Harukazu Fukami
1Suntory Biomedical Research Limited, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka, Japan.
Summary
Mast cell chymase contributes to contact dermatitis by increasing eosinophil infiltration. This finding suggests chymase may play a role in chronic skin conditions like atopic dermatitis.
Area of Science:
- Immunology
- Dermatology
- Enzymology
Background:
- Contact dermatitis is an inflammatory skin condition.
- Mast cell chymase is a protease implicated in allergic inflammation.
- Eosinophils are immune cells involved in allergic responses.
Purpose of the Study:
- To investigate the role of mast cell chymase in 2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis.
- To determine if chymase activity contributes to skin swelling and eosinophil infiltration.
- To explore the potential involvement of chymase in chronic dermatitis pathogenesis.
Main Methods:
- Epicutaneous application of DNFB to mouse ears to induce dermatitis.
- Administration of a chymase inhibitor to assess its effect on dermatitis.
- Intradermal injection of human chymase into mouse ears.
- In vitro assays to measure chymase-induced eosinophil migration.
Main Results:
- DNFB-induced dermatitis and eosinophil infiltration were observed.
- Chymase inhibition significantly reduced ear swelling and eosinophil accumulation.
- Direct injection of human chymase caused skin swelling and eosinophil infiltration.
- Chymase stimulated eosinophil migration in a concentration-dependent manner in vitro.
Conclusions:
- Mast cell chymase plays a significant role in DNFB-induced contact dermatitis.
- Chymase likely promotes dermatitis by enhancing eosinophil infiltration.
- Chymase may be a key factor in the pathogenesis of chronic inflammatory skin diseases, including atopic dermatitis.