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Growth inhibitory effect of paradicsompaprika in cancer cell lines
Takeshi Mori1, Masafumi Ohnishi, Megumi Komiyama
1Department of Hospital Pharmacy, Aichi Medical University School of Medicine, Nagakute-cho, Aichi-gun, Aichi 480-1195, Japan. 100019@gk.amu.aichi-med-u.ac.jp
Abstract:
We investigated whether components of paradicsompaprika have direct antitumor effects or inhibitory effects on cancer growth, using its water extract. We applied collagen gel droplet embedded culture drug sensitivity test (CD-DST) as a screening method, which was developed based on the characteristics of cell culture on collagen matrix. Colon adenocarcinoma cells, epithelial cells of lung cancer, and cervical cancer cells were used. Paradicsompaprika is classified as Capsiucum annume L. var. grossum of Solanaceae. It is the first of the Hungarian species that was planted in Japan. It is available as TOMA-P in Japan. TOMA-P contains abundant carotenoids including capsanthin and beta-carotene. Water extract of paradicsompaprika was added to each cell at each concentration, and the mixture was cultured for 24 h and 7 days. The inhibitory effects against lung cancer and cervical cancer were observed concentration- and time-dependently. The effect was more prominent against lung cancer. The growth of bowel cancer cells was observed after the 7-day exposure of paradicsompaprika at the concentrations below the highest concentration compared to the control. At the highest concentration, the growth inhibition was not different between the 24-h exposure and the 7-day exposure, which suggests that tumor dormancy was induced. Results of the present study suggest that the water extract of paradicsompaprika can be a candidate of a new anticancer agent. Fat soluble component of paradicsompaprika, capsanthin is regarded as an anti-promoter of cancer. Thus, paradicsompaprika possesses chemopreventive and inhibitory effects on cancer cells.
Insights
Water extract from paradicsompaprika (TOMA-P) shows anticancer potential, inhibiting lung and cervical cancer cell growth. It may also induce tumor dormancy in bowel cancer cells, suggesting chemopreventive properties.
Area of Science:
- Oncology
- Pharmacology
- Plant-based medicine
Background:
- Paradicsompaprika (Capsicum annuum L. var. grossum), known as TOMA-P in Japan, is rich in carotenoids like capsanthin and beta-carotene.
- Its potential therapeutic properties, particularly in cancer, have not been extensively studied.
Purpose of the Study:
- To investigate the direct antitumor and cancer growth inhibitory effects of paradicsompaprika water extract.
- To evaluate the efficacy of paradicsompaprika extract against lung, cervical, and colon adenocarcinoma cells.
Main Methods:
- Utilized the collagen gel droplet embedded culture drug sensitivity test (CD-DST) for drug screening.
- Applied water extract of paradicsompaprika to lung, cervical, and colon cancer cell lines at various concentrations and incubation times (24 hours and 7 days).
Main Results:
- Observed concentration- and time-dependent inhibition of lung and cervical cancer cell growth, with a more pronounced effect on lung cancer.
- Colon adenocarcinoma cell growth was observed at lower concentrations after 7 days, but growth inhibition was noted at the highest concentration, suggesting tumor dormancy.
- Capsanthin, a fat-soluble component, is recognized for its anti-promoter effects on cancer.
Conclusions:
- Paradicsompaprika water extract demonstrates potential as a novel anticancer agent.
- The plant exhibits both chemopreventive and cancer inhibitory effects on various cancer cell types.