Efficient embedding technique for preparing small specimens for stereological volume estimation: zebrafish larvae
Related Experiment Video
Updated: Sep 30, 2026
Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
Published on: October 17, 2018
A J Hill1, C V Howard, A R Cossins
1Developmental Toxico-Pathology Research Group, Department of Human Anatomy and Cell Biology, Faculty of Medicine, Ashton Street, University of Liverpool, Liverpool L69 3GE, UK. a.j.hill@liv.ac.uk
Abstract:
Stereological sampling regimes, in particular volume and number estimation, often require systematic uniformly random sections throughout a specimen. A method has been developed to increase the efficiency of preparing fish larvae for sectioning prior to histological or stereological analysis. Embedding a group of larvae in a resin block using this technique greatly reduces the quantity of sections produced and allows easy assessment of sample groups. Saving time in this way therefore makes stereology a more viable research tool.
