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A new lipophilic fluorescent probe for interaction studies of bioactive lipopeptides with membrane models
Elisa Peroni1, Gabriella Caminati, Piero Baglioni
1Dipartimento di Chimica Organica Ugo Schiff, Università degli Studi di Firenze, Polo Scientifico, I-50019 Sesto Fiorentino (FI), Italy.
Bioorganic & Medicinal Chemistry Letters
|June 18, 2002
Abstract:
The new fluorescent lipophilic moiety 11-[(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-acetyl)amino]undecanoic acid (AMCA-omegaAud-OH) was introduced by SPPS at the N-terminus of the immunodominant epitope GpMBP(74-85). FRET experiments using the new fluorescent lipopeptide demonstrate that the peptide interacts with much more affinity with the membrane compared to the lipid free analogue.