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Arabidopsis map-based cloning in the post-genome era
Georg Jander1, Susan R Norris, Steven D Rounsley
1Cereon Genomics LLC, 45 Sidney Street, Cambridge, MA 02139, USA. georg.jander@cereon.com
Plant Physiology
|June 18, 2002
Summary
Map-based cloning identifies genetic causes of mutant phenotypes. Advances in Arabidopsis genomics and polymorphism detection make this process routine, exemplified by VTC2 gene cloning.
Area of Science:
- Plant genetics and genomics
- Molecular biology
- Arabidopsis research
Background:
- Map-based cloning is a powerful genetic analysis technique.
- It identifies genes responsible for mutant phenotypes without prior assumptions.
- Recent advances have streamlined map-based cloning in Arabidopsis.
Purpose of the Study:
- To describe the Cereon Arabidopsis Polymorphism Collection.
- To present a generic approach for mutation mapping in Arabidopsis using this collection.
- To demonstrate the utility of this approach by cloning the VTC2 gene.
Main Methods:
- Utilizing the Cereon Arabidopsis Polymorphism Collection, a resource of over 50,000 markers.
- Employing improved DNA polymorphism detection methods.
- Applying a generic mutation mapping strategy in Arabidopsis.
Main Results:
- The Cereon Collection facilitates routine map-based cloning in Arabidopsis.
- A generic mutation mapping approach was successfully applied.
- The VTC2 gene was successfully mapped and cloned using this method.
Conclusions:
- Map-based cloning in Arabidopsis is now more accessible due to genomic sequencing and marker availability.
- The Cereon Collection provides a valuable resource for genetic studies in Arabidopsis.
- This approach enables efficient identification of genes underlying mutant phenotypes.