Related Experiment Video
Updated: Aug 3, 2026

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
Published on: June 28, 2019
[Comparison of the homologous SfeI and LlaBI restriction-modification systems]
S S Okhapkina1, N A Netesova, L N Golikova
1Institute of Molecular Biology, Vektor State Research Center of Virology and Biotechnology, Kol'tsovo, Novosibirsk Region, 633159 Russia. sso@lae.ma.nsc.ru
Abstract:
A fragment containing the SfeI restriction-modification system (RMS) operon was cloned from a Streptococcus faecalis SE72 plasmid. Nucleotide sequence analysis revealed its high (99.2%) homology to the operon for Lactococcus lactis subsp. cremoris W56 LlaBI RMS recognizing the same site, 5'-CTRYAG-3'. A substantial difference was that SfeI RMS operon had an additional 198-bp fragment and a larger gene for the putative control protein. No homology was observed between operon-flanking sequences of the two closely related species, suggesting horizontal transfer of the operon.
More Related Videos
Related Concept Videos
Homologous Recombination
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Cis-regulatory Sequences
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
CRISPR/Cas9 Genome Editing

