Expression and behaviour of CENP-E at kinetochores during mouse spermatogenesis

Centromere protein E (CENP-E) is a microtubule motor protein localised in the outer kinetochore plate and in the fibrous corona that relocalises to the midzone in early anaphase. While its expression in somatic cells has been widely analysed, an accurate description of its behaviour during the two meiotic divisions has not yet been reported. We have carefully analysed by immunofluorescence the subcellular distribution of CENP-E during mouse spermatogenesis. CENP-E first appears during late diakinesis/early prometaphase I as very bright C-shaped or "crescent" signals at each homologous centromere. These crescent CENP-E signals are also observed in unaligned prometaphase I bivalents that are not attached to spindle microtubules, while in bioriented metaphase I bivalents two kinds of fainter signals are observed. Thus, some bivalents present a plate-like signal while others show a pair of spots representing sister kinetochores at each homologous centromere. Double labelling of CENP-E with CENP-G and an anti-centromere serum indicates that in meiosis CENP-E is also located at the outer kinetochore plate and the fibrous corona. During early anaphase I CENP-E relocalises from kinetochores to the midzone where it is detected as fibrous strands, although some residual labelling persists at kinetochores until telophase I. During this stage CENP-E is detected as two parallel plates at the intercellular bridge. The general pattern of labelling during meiosis II is similar to that found during meiosis I. Our results suggest that CENP-E is implicated in the spindle checkpoint, and in chromosome alignment, during the two meiotic divisions in vertebrate males. We also demonstrate that the centromere changes its structure once alignment of all bivalents at the metaphase I plate has been reached.