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CD56 is expressed exclusively on CD3+ T lymphocytes in canine peripheral blood
Isao Otani1, Tsuyoshi Niwa, Masazumi Tajima
1Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Insights
Canine peripheral blood lymphocytes express CD56, a neural cell adhesion molecule. These CD56-positive cells are a subset of T lymphocytes, with distinct CD4 and CD8 co-receptor expressions.
Area of Science:
- Immunology
- Veterinary Immunology
- Canine Leukocyte Biology
Background:
- CD56 is a neural cell adhesion molecule.
- Its expression on canine leukocytes is not well-characterized.
- Understanding leukocyte subsets is crucial for canine health research.
Purpose of the Study:
- To characterize CD56+ cells in healthy adult beagle dogs.
- To determine the immunophenotype of CD56+ canine leukocytes.
- To investigate the expression of CD56 on canine T lymphocyte subsets.
Main Methods:
- Flow-cytometric analysis of peripheral blood from 30 healthy adult beagle dogs.
- Utilized anti-human CD56 antibody (Leu-19) for detection.
- Phenotypic analysis included CD3, CD4, and CD8 co-receptor expression.
Main Results:
- CD56+ cells were identified in 19 out of 30 dogs, comprising 8.8-21.7% of peripheral blood lymphocytes.
- All CD56+ cells also expressed CD3 molecules.
- Subsets included CD4-CD8+CD56+ (50.6%) and CD4+CD8-CD56+ (43.7%) cells.
- CD56 expression intensity was significantly higher on CD4-CD8+CD56+ cells.
Conclusions:
- CD56 is uniquely expressed on subsets of T lymphocytes in canine peripheral blood.
- These findings contribute to the understanding of canine immune cell populations.
- The characterized CD56+ T cells represent a novel subset in dogs.
Abstract:
CD56+ cells in canine blood leukocytes were characterized by flow-cytometric analysis of peripheral blood of 30 healthy adult beagle-dogs (15 males and 15 non-pregnant females). In 19 of the 30 dogs, anti human CD56 antibody, Leu-19, reacted with 8.8-21.7% of peripheral blood lymphocytes. All CD56+ cells simultaneously expressed CD3 molecules on their surface. Further phenotypic analysis revealed that 50.6+/-13.1% of the CD56+ cells showed CD4-CD8+ phenotype and 43.7+/-10.1% showed CD4+CD8- phenotype. Expression intensity of CD56 on the CD4-CD8+CD56+ cells was significantly higher than that on CD4+CD8-CD56+ cells (P<0.001). These findings indicate that CD56, which is a neural cell adhesion molecule, is uniquely expressed on subsets of T lymphocytes in canine peripheral blood.