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Updated: Aug 15, 2026

Influenza Virus Propagation in Embryonated Chicken Eggs
Published on: March 19, 2015
In vitro effects of some steroidal hormones on the replication of avian pneumovirus
A I Al-Afaleq1, R C Jones, A M Homeida
1Department of Microbiology and Parasitology, College of Veterinary Medicine and Animal Resources, King Faisal University, Al Ahsa, Saudi Arabia.
Abstract:
The effects of testosterone, oestradiol, progesterone and cortisone on the in vitro replication of avian pneumovirus in tracheal organ cultures (TOC) were investigated. Samples of cell-associated and cell-free virus from TOC, grown in medium containing these hormones, were taken at selected intervals. Progesterone and cortisone caused a slight increase in cell-associated virus. Testosterone and oestradiol caused a slight delay and decrease in virus replication when compared with the controls. All groups shared the same time interval to reach peak cell-free virus titre, 96 h post inoculation. In comparison with the controls, only a small drop (0.25-0.50 log10) in the peak of virus titre was observed in the hormone treated groups.
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