Related Experiment Video
Updated: Sep 30, 2026

A Low Mortality Rat Model to Assess Delayed Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage
Published on: January 17, 2013
Vasospasm following subarachnoid hemorrhage: evidence against functional upregulation of Rho kinase constrictor
Seonghun Yoon1, Jonathan D Sherman, Mario Zuccarello
1Department of Pharmacology and Cell Biophyscis, University of Cincinnati College of Medicine, OH 45267-0575, USA.
Abstract:
This study tested the hypothesis that vasospasm due to subarachnoid hemorrhage involves the functional upregulation of Rho kinase. Spasm of the rabbit basilar artery was achieved using a double hemorrhage model, which we previously demonstrated was endothelin-1 dependent. In situ effects of agents were determined by direct measurement of vessel diameter following their suffusion in a cranial window. Y-27632, a Rho kinase inhibitor, relaxed the spasm. However, relaxations to Y-27632 were not significantly greater in endothelin-1 constricted spastic vessels initially relaxed with the endothelin converting enzyme inhibitor, phosphoramidon, as compared to endothelin-1 constricted control vessels. These results suggest that, at least in the rabbit double subarachnoid hemorrhage model, vasospasm does not involve the functional upregulation of Rho kinase.
Related Concept Videos
Vascular Spasm
Hemorrhagic Stroke ll: Pathophysiology
Cerebral Edema ll: Pathophysiology
Autoregulation of Blood Flow
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.
Hemorrhagic Stroke l: Introduction
