Detection of aminotransferase activity of Propionibacterium freudenreichii after SDS-PAGE
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Updated: Sep 30, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
Anne Thierry1, Marie-Bernadette Maillard, Sylvie Lortal
1Laboratoire de Recherches de Technologie Laitière, I.N.R.A., 65 rue de Saint-Brieuc, 35042 Rennes Cedex, France. athierry@rennes.inra.fr
Abstract:
Aminotransferases (ATs) had previously been detected after native electrophoresis. We show now that aminotransferase(s) of Propionibacterium freudenreichii can be detected after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Moreover, it retained a high activity (84%) in the presence of 0.23% SDS, contrary to what was observed for aminotransferase(s) of Bifidobacterium bifidum (54%) and of six other cheese-related species (0-20%).

