Related Experiment Video
Updated: Sep 30, 2026

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Published on: December 30, 2021
Thermophilic protease-producing Geobacillus from Buranga hot springs in Western Uganda
Joseph F Hawumba1, Jacques Theron, Volker S Brözel
1Biochemistry Department, Makerere University, P.O. Box 7062, Kampala, Uganda.
Abstract:
Two proteolytic thermophilic aerobic bacterial strains, PA-9 and PA-5, were isolated from Buranga hot springs in western Uganda. The cells were rods, approximately 10-12 microm in length and 3 microm in width. Isolate PA-9 grew at between 38 degrees C and 68 degrees C (optimum, 62 degrees C), and PA-5 grew at between 37 degrees C and 72 degrees C (optimum, 60 degrees C). Both isolates grew optimally at pH 7.5-8.5. Their 16S rRNA gene sequences indicated that they belong to the newly described genus Geobacillus. Zymogram analysis of the crude enzyme extracts revealed the presence of two extracellular proteases for isolate PA-5, and at least eight for isolate PA-9. The optimum temperature and pH for casein-degrading activity were 70 degrees C, pH 6.5 for isolate PA-9, but caseinolytic activity could also be observed at 2 degrees C. In the case of isolate PA-5, optimal activity was observed over a temperature and pH range of 50-70 degrees C and pH 5-10, respectively.
More Related Videos
12:23Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
08:11Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius
Published on: June 14, 2024
Related Concept Videos
Hyperthermophilic Bacteria
Diversity of Archaea IV
Diversity of Archaea I
Factors Influencing Microbial Growth: Temperature
Diversity of Archaea III
Anoxygenic Phototrophic Bacteria