Related Experiment Video

Updated: Aug 10, 2026

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
11:00

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Published on: September 10, 2010

Applications of adhesion molecule gene knockout cell lines

Jordan A Kreidberg1

  • 1Department of Medicine, Division of Nephrology, Children's Hospital, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA.

Methods in Cell Biology
|June 20, 2002
PubMed

Insights

Mouse cell lines with gene mutations are valuable for studying cell adhesion and extracellular matrix (ECM) gene functions in development. These models reveal novel phenotypes not seen in whole organisms, aiding molecular function research.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Cell lines with targeted mutations in adhesion or ECM genes are crucial research tools.
  • ES cell lines homozygous for mutations offer insights into gene function.

Purpose of the Study:

  • To highlight the utility of mouse-derived cell lines for studying gene function.
  • To emphasize the value of in vitro models for uncovering novel phenotypes.

Main Methods:

  • Utilizing mouse cell lines with targeted mutations in adhesion or ECM genes.
  • Employing ES cell lines homozygous for specific gene mutations.
  • In vitro experimentation to analyze molecular functions.

Main Results:

  • Cell lines revealed phenotypes not apparent from in vivo studies.
  • In vitro systems demonstrated higher dependence on specific molecules compared to whole organisms.
  • Identified novel functions of adhesion receptors.

Conclusions:

  • Mouse cell lines with targeted mutations are essential for understanding gene function in development.
  • In vitro models provide unique insights into molecular mechanisms.
  • These cell lines will continue to be vital for studying adhesion receptor functions.

More Related Videos

Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments12:34

Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments

Published on: September 16, 2016

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells09:37

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Published on: August 25, 2021

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...