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The nanoleakage phenomenon: influence of moist vs dry bonding
Thomas Pioch1, Hans Jörg Staehle, Marcus Wurst
1Department of Restorative Dentistry, University of Heidelberg, Germany. thomas_pioch@med.uni-heidelberg.de
Purpose:
To assess the degree of nanoleakage of Class V preparations restored with three bonding agents using wet and dry bonding.
Materials And Methods:
Standardized Class V cavities were prepared in 66 human molars with cervical margins located in dentin. The composite was placed using one of three different dentin bonding agents with a moist or dry bonding technique (n = 11 per group). The teeth were stored in a 1% rhodamin-B solution for 24 h at 20 degrees C, then rinsed with water, embedded in methacrylate, and sectioned parallel to the long axis of the tooth, separating the restorations into two parts. A confocal laser scanning microscope (CLSM) was used to visualize the layer 10 microm under the prepared surface of the section. The lengths of the dye-penetrated pathways were measured, representing the amount of nanoleakage.
Results:
Differences in nanoleakage between the tested materials were proven to be statistically significant. The effect of drying had a significant influence on both the acetone-based and the ethanol-based material, but not on the water-based bonding resin.
Conclusion:
Dentin drying may have an influence on nanoleakage, depending upon the nature of the individual bonding agent.