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Related Experiment Videos

T-helper cells from naive to committed.

Armin Saalmüller1, Tobias Werner, Vicky Fachinger

  • 1Institute for Immunology, Federal Research Centre for Virus Diseases of Animals, P.O. Box 1149, Paul-Ehrlich-Strasse 28, 72076 Tübingen, Germany. armin.saalmueller@tue.bfav.de

Veterinary Immunology and Immunopathology
|June 20, 2002
PubMed
Summary

In pigs, naive CD4(+)CD8(-) T-helper cells mature into CD4(+)CD8alpha(+) memory cells. This CD4(+)CD8alpha(+) subpopulation is crucial for secondary immune responses, particularly against classical swine fever virus.

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Area of Science:

  • Immunology
  • Swine immunology
  • Cellular immunology

Background:

  • T-helper cells are vital for adaptive immunity in pigs.
  • Two distinct CD4(+) T-helper cell subpopulations exist in extra-thymic compartments.
  • These subpopulations differ in surface antigen expression, including CD8alpha and MHCII.

Purpose of the Study:

  • To investigate the differentiation and function of porcine T-helper cell subpopulations.
  • To identify the phenotype of naive and memory T-helper cells in swine.
  • To understand the role of CD4(+)CD8alpha(+) T-cells in secondary immune responses.

Main Methods:

  • In vitro immune reactions stimulated with Staphylococcal Enterotoxin B (SEB).
  • Analysis of surface antigen expression (CD4, CD8alpha, MHCII, CD45RC) on T-helper cells.

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  • Secondary in vitro immune response against classical swine fever virus.

    • Main Results:

    • Primary immune response mainly involves the CD4(+)CD8(-) subpopulation (naive T-helper cells).
    • Activation leads to CD8alpha and MHCII expression and CD45RC down-regulation, resembling the CD4(+)CD8alpha(+) subpopulation.
    • Only the CD4(+)CD8alpha(+) T-helper subpopulation responded in secondary immune assays, indicating it harbors memory cells.

    Conclusions:

    • Naive CD4(+)CD8(-)MHCII(-) porcine T-helper cells undergo extra-thymic maturation.
    • This maturation results in committed CD4(+)CD8alpha(+)MHCII(+) T-helper cells.
    • The CD4(+)CD8alpha(+) subpopulation represents T-helper memory cells in swine.