Classical mutagenesis techniques

Christopher W Lawrence¹

¹Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.

Methods in Enzymology

|June 21, 2002

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Classical mutagenesis techniques

Related Concept Videos

Following the RAD6 pathway.

The Saccharomyces cerevisiae rev6-1 mutation, which inhibits both the lesion bypass and the recombination mode of DNA damage tolerance, is an allele of POL30, encoding proliferating cell nuclear antigen.

The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination.

Transient loss of MHC class I tetramer binding after CD8+ T cell activation reflects altered T cell effector function.

Binding of MutS mismatch repair protein to DNA containing UV photoproducts, "mismatched" opposite Watson--Crick and novel nucleotides, in different DNA sequence contexts.

Frequency, specificity, and sites of expansion of CD8+ T cells during primary pulmonary influenza virus infection.

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Evolutionary genetic approaches to analyze mucins.

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Related Experiment Videos

Classical mutagenesis techniques

Related Concept Videos

Following the RAD6 pathway.

The Saccharomyces cerevisiae rev6-1 mutation, which inhibits both the lesion bypass and the recombination mode of DNA damage tolerance, is an allele of POL30, encoding proliferating cell nuclear antigen.

The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination.

Transient loss of MHC class I tetramer binding after CD8+ T cell activation reflects altered T cell effector function.

Binding of MutS mismatch repair protein to DNA containing UV photoproducts, "mismatched" opposite Watson--Crick and novel nucleotides, in different DNA sequence contexts.

Frequency, specificity, and sites of expansion of CD8+ T cells during primary pulmonary influenza virus infection.

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Classical mutagenesis techniques

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Following the RAD6 pathway.

The Saccharomyces cerevisiae rev6-1 mutation, which inhibits both the lesion bypass and the recombination mode of DNA damage tolerance, is an allele of POL30, encoding proliferating cell nuclear antigen.

The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination.

Transient loss of MHC class I tetramer binding after CD8+ T cell activation reflects altered T cell effector function.

Binding of MutS mismatch repair protein to DNA containing UV photoproducts, "mismatched" opposite Watson--Crick and novel nucleotides, in different DNA sequence contexts.

Frequency, specificity, and sites of expansion of CD8+ T cells during primary pulmonary influenza virus infection.

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Ex vivo imaging and enzymatic analysis of intestinal mucus.

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Related Experiment Videos

Classical mutagenesis techniques

Related Concept Videos

Related Articles

Following the RAD6 pathway.

The Saccharomyces cerevisiae rev6-1 mutation, which inhibits both the lesion bypass and the recombination mode of DNA damage tolerance, is an allele of POL30, encoding proliferating cell nuclear antigen.

The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination.

Transient loss of MHC class I tetramer binding after CD8+ T cell activation reflects altered T cell effector function.

Binding of MutS mismatch repair protein to DNA containing UV photoproducts, "mismatched" opposite Watson--Crick and novel nucleotides, in different DNA sequence contexts.

Frequency, specificity, and sites of expansion of CD8+ T cells during primary pulmonary influenza virus infection.

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Evolutionary genetic approaches to analyze mucins.

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.