Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vasodilating procyanidins derived from grape seeds.

David F Fitzpatrick1, Bettye Bing, David A Maggi

  • 1Department of Pharmacology, College of Medicine, University of South Florida, Tampa, Florida 33612-4799, USA. dfitzpat@hsc.usf.edu

Annals of the New York Academy of Sciences
|June 21, 2002
PubMed
Summary

Grape seed extracts contain procyanidins that promote cardiovascular health by releasing nitric oxide (NO), a key molecule in blood vessel relaxation. These compounds may help prevent or treat cardiovascular diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rare variants in NR2F2 cause congenital heart defects in humans.

American journal of human genetics·2014
Same author

Strategies and data analysis techniques for lipid and phospholipid chemistry elucidation by intact cell MALDI-FTMS.

Journal of the American Society for Mass Spectrometry·2004
Same author

Use of double-depleted 13C and 15N culture media for analysis of whole cell bacteria by MALDI time-of-flight and Fourier transform mass spectrometry.

Journal of the American Society for Mass Spectrometry·2003
Same author

Investigation of MALDI-TOF and FT-MS techniques for analysis of Escherichia coli whole cells.

Analytical chemistry·2003

Area of Science:

  • Cardiovascular Science
  • Natural Product Chemistry
  • Pharmacology

Background:

  • Grape seed extracts (GSE), skins, juice, and red wines exhibit endothelium-dependent relaxing (EDR) activity.
  • This activity is mediated by endothelial nitric oxide (NO) release, impacting vascular smooth muscle cells via the cyclic GMP pathway.
  • The NO/cyclic GMP pathway plays a crucial role in cardiovascular protection.

Purpose of the Study:

  • To isolate and identify the specific procyanidin compounds responsible for EDR activity in GSE.
  • To characterize the relationship between procyanidin structure and EDR activity.

Main Methods:

  • Methanol extraction of crushed Concord grape seeds followed by separation into seven fractions using Toyopearl TSK-HW-40 chromatography.
  • High-performance liquid chromatography (HPLC) to separate EDR-active fractions into individual compound peaks.

Related Experiment Videos

  • Identification of procyanidins using electrospray-ion trap mass spectrometry (ES-ITMS), MS/MS, tannase treatment, and acid thiolysis.

    • Main Results:

    • Sixteen individual compounds exhibiting EDR activity were identified from fractions D-G.
    • EDR activity of isolated procyanidins correlated positively with their degree of polymerization, epicatechin content, and galloylation.
    • The threshold for relaxation varied, ranging from less than 0.5 microg/mL to greater than 4 microg/mL.

    Conclusions:

    • Procyanidins are the primary EDR-active compounds in GSE.
    • These identified procyanidins, possessing antioxidant properties, may serve as therapeutic agents for cardiovascular diseases.
    • Grape-derived products like wines, juices, and supplements containing these compounds could be beneficial for cardiovascular health.