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[Fungal origin of PBV-group viruses isolated from Penicillium brevi-compactum]
Abstract:
Treatment of extracts from the disintegrated mycelium of Penicillium brevi-compactum with antisera against viruses PBV-1 and PBV-3 grown on bacteria results in a considerable decrease in the infectiveness of micellar preparations toward E. coli C. This is a specific reaction since treatment with a heterologic antiserum against phage T2 has no effect on the infectiveness of the micellar extracts. The kinetics of reassociation of DNA from PBV-3 and DNA from Penicillium brevi-compactum has shown that the value of Cot at half-reassociation of these DNAs is 13.3 times higher than the value of Cot at half-reassociation of DNA from PBV-3 and heterologic DNA from chicken embryos. As was found by calculations, DNA isolated from the fungal mycelium contains virus sequences at an amount of 3.7 virus genomes per cell. These data confirm the micellar origin of the viruses PBV-1 and PBV-3. The virus PBV-3 is supposed to be present in the cells in the form of prophage.
Insights
Penicillium brevi-compactum viruses (PBV-1 and PBV-3) were confirmed to originate from the fungus. Virus PBV-3 may exist as a prophage within the fungal cells.
Area of Science:
- Mycology
- Virology
- Molecular Biology
Context:
- Fungal viruses can impact host cell biology and genetic material.
- Understanding virus-fungus interactions is crucial for microbial genetics.
Purpose:
- To investigate the origin of Penicillium brevi-compactum viruses (PBV-1 and PBV-3).
- To determine if these viruses are integrated within the fungal genome.
Summary:
- Antisera against PBV-1 and PBV-3 reduced the infectivity of Penicillium brevi-compactum extracts on E. coli C, indicating a specific interaction.
- DNA reassociation kinetics revealed that PBV-3 DNA sequences are present in Penicillium brevi-compactum DNA, suggesting viral integration.
- Calculations indicated approximately 3.7 viral genomes per fungal cell, supporting the micellar origin of the viruses.
Impact:
- Provides evidence for the endogenous origin of PBV-1 and PBV-3 viruses within Penicillium brevi-compactum.
- Suggests that PBV-3 may exist in a prophage state within the fungal mycelium.
- Contributes to the understanding of virus-host interactions in fungi.