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CD14 gene -159C/T polymorphism is not associated with coronary artery disease and myocardial infarction
Werner Koch1, Adnan Kastrati, Julinda Mehilli
1Deutsches Herzzentrum München and 1 Medizinische Klinik rechts der Isar, Technische Universität München, Germany. wkoch@dhm.mhn.de
Insights
The CD14 gene
Area of Science:
- Genetics and Molecular Biology
- Cardiovascular Medicine
- Immunology
Background:
- Monocyte differentiation antigen CD14 is a key mediator in inflammatory responses linked to atherosclerosis, coronary artery disease (CAD), and myocardial infarction (MI).
- A specific C to T nucleotide substitution polymorphism at position -159 in the CD14 gene promoter was investigated as a potential risk factor.
Purpose of the Study:
- To determine if the CD14 gene -159C/T polymorphism is associated with an increased risk of developing coronary artery disease (CAD) or myocardial infarction (MI).
Main Methods:
- Genotyping for the CD14 -159C/T polymorphism was performed in patients with documented CAD (n=998) and MI (n=793).
- Control groups included subjects without CAD (n=340) and healthy blood donors (n=104), matched for age and gender.
- Statistical analysis was conducted to compare genotype distributions and assess associations after adjusting for cardiovascular risk factors.
Main Results:
- Genotype distributions (CC:CT:TT) for the -159C/T polymorphism were similar across all study groups, including controls, CAD patients, and MI patients.
- No significant association was found between the CD14 -159C/T polymorphism and the risk of CAD or MI, even after adjusting for conventional cardiovascular risk factors.
- Subgroup analyses also revealed no significant differences in genotype distributions between control subjects and patients with CAD or MI.
Conclusions:
- The -159C/T polymorphism in the CD14 gene is not associated with coronary artery disease (CAD) or myocardial infarction (MI) in the studied patient population.
- These findings suggest that this specific genetic variation in CD14 does not contribute to the risk of developing these cardiovascular conditions.
Background:
Monocyte differentiation antigen CD14 is considered an important cell-activating mediator of inflammatory responses that may result in atherosclerosis, coronary artery disease (CAD), thrombus formation, and myocardial infarction (MI). We assessed the possibility that a C --> T nucleotide substitution polymorphism in the promoter (position -159) of the gene encoding CD14 constitutes a risk factor for CAD and MI.
Methods:
Consecutive patients with significant, angiographically documented coronary stenoses but without symptoms or signs of old or acute MI constituted the group with CAD (n = 998). Consecutive patients with angiographic examination with old or acute MI constituted the group with MI (n = 793). Subjects matched with patients for age and gender but without angiographic evidence of CAD and without symptoms or signs of MI (n = 340) and a group of healthy blood donors (n = 104) served as controls.
Results:
Genotype distributions of the -159C/T polymorphism were similar across the groups; CC:CT:TT was 26.9%:51.0%:22.1% in blood donors, 25.9%:52.0%:22.1% in matched control subjects, 27.4%:49.9%:22.7% in patients with CAD, and 29.2%:49.2%:21.6% in patients with MI. The lack of association persisted also after adjustment for the presence of conventional cardiovascular risk factors. In addition, no significant differences were found between genotype distributions of control subjects and selected subgroups of patients with CAD or MI.
Conclusion:
These findings indicate that, in the sample of patients examined in this study, the -159C/T polymorphism of the CD14 gene is not related to CAD or MI.