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Kinetics of Ionizing Irradiation-induced DNA Strand Breaks
Chun-Lin Shao1, Masahiro Saito, Zeng-Liang Yu
1Institute of Plasma Physics, Chinese Academy of Sciences, P O Box 1126, Hefei 230031, China. clshao@mail.ipp.ac.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|June 21, 2002
Abstract:
By measuring the dose effect of ionizing irradiation-induced single- and double-strand breaks (SSB and DSB) of pBR322, the responses of the yields of SSB and DSB to DNA concentrations were obtained. It was found that, in the presence of mannitol, the reciprocals of G(SSB) and G(alphaDSB) had a linear relationship to the reciprocal of DNA concentration so that the competition reaction of DNA and mannitol to.OH radicals could be described by a second-order kinetics. Moreover, the rate coefficients and the efficiencies of the.OH radical inducing SSB and alphaDSB were deduced.