Deinococcus radiodurans CatB Gene Cloning and Expression in Escherichia coli

A 1 611 bp length complete coding sequence of the catalase (Cat) was obtained through bioimformatical analysis of the database of D.radiodurans genome, and then was amplified from D. radiodurans genomic DNA by polymerase chain reaction. The amplified gene was cloned into pKK223-3 vector and transformed into E.coli UM2, a Cat-deficient mutant. Staining of non-denaturing polyacrylamide gels for Cat activity demonstrated that pKK223-3 insert encoded a Cat that co-migrated with the CatB found in D.radiodurans cell lysates. Expression of CatB gene from D.radiodurans in E.coli UM2 restored the resistance to H(2)O(2) at low concentrations.