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Related Concept Videos

Ion Channels01:19

Ion Channels

The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Thermosensation01:43

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

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Related Experiment Video

Updated: May 10, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

TRPV3 is a calcium-permeable temperature-sensitive cation channel.

Haoxing Xu1, I Scott Ramsey, Suhas A Kotecha

  • 1Howard Hughes Medical Institute, Children's Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA.

Nature
|June 22, 2002
PubMed
Summary

Researchers identified human TRPV3 (hTRPV3), a novel thermosensitive channel. This protein functions in the physiological temperature range (22–40°C), expanding our understanding of temperature sensation.

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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

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Last Updated: May 10, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Area of Science:

  • Molecular biology
  • Neuroscience
  • Physiology

Background:

  • Transient receptor potential (TRP) proteins are crucial cation channels involved in sensation and vasoregulation.
  • Known TRP channels are activated by specific temperature ranges (e.g., TRPV1 by heat, TRPM8 by cold), but the 22–40°C range remains under-explored.

Purpose of the Study:

  • To identify molecular determinants of thermosensation within the physiological temperature range of 22–40°C.
  • To characterize a novel vanilloid channel family member involved in temperature sensing.

Main Methods:

  • Cloning and expression of human TRPV3 (hTRPV3) in mammalian cells.
  • Measurement of intracellular calcium levels and nonselective cationic conductance in response to temperature changes.
  • Analysis of temperature dependence, sensitization, and hysteresis of the channel activity.

Main Results:

  • hTRPV3 was identified and found to be expressed in various tissues, including skin, tongue, and nervous system components.
  • Elevated intracellular calcium and nonselective cationic conductance were observed in hTRPV3-transfected cells when temperatures increased from 22°C to 40°C.
  • The channel activity exhibited steep temperature dependence, sensitization upon repeated heating, and hysteresis during temperature cycling.

Conclusions:

  • hTRPV3 is a thermosensitive channel activated within the physiological temperature range (22–40°C).
  • This finding proposes hTRPV3 as a key player in sensing temperatures between those detected by TRPM8 and TRPV1.
  • The study expands the known repertoire of thermosensitive TRP channels and their roles in physiological processes.