Related Experiment Videos
Engineering a composite neotrachea with surgical adhesives
Edward J Doolin1, Louise F Strande, Xialoi Sheng
1Division of Pediatric Surgery Department, Robert Wood Johnson Medical School at Camden, Camden, NJ, USA.
Journal of Pediatric Surgery
|June 22, 2002
Summary
Biologic surgical adhesive TISSEEL effectively fused engineered cartilage and bronchial epithelium. The composite tissue remained viable and morphologically accurate, showing promise for bioengineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Reconstructive surgery is often limited by normal tissue availability.
- Tissue engineering offers potential for creating "artificial tissues."
Purpose of the Study:
- To evaluate the efficacy and viability of TISSEEL, a biologic surgical adhesive.
- To assess TISSEEL's ability to combine engineered bronchial epithelium with engineered cartilage.
Main Methods:
- Engineered human bronchial epithelium and mature cartilage from isolated cells.
- Utilized TISSEEL for biologic fusion of the engineered tissues.
- Cultured the fused composite for 5 days, assessing mechanical properties and morphology.
Main Results:
- TISSEEL successfully fused the engineered epithelium to cartilage.
- The composite construct demonstrated viability for 5 days in tissue culture.
- No significant changes in bronchial epithelium cell size or thickness were observed.
Conclusions:
- Biologically compatible fibrin glue (TISSEEL) is an effective surgical adhesive for tissue fusion.
- Fused tissues maintained viability and morphological accuracy.
- Surgical adhesives show promise for developing composite tissues in bioengineering.