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Related Experiment Video

Updated: Jun 24, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

[Reviews for Gram stain].

Kiyoharu Yamanaka¹

¹Department of Clinical Laboratory, Otemae Hospital, Osaka 540-0008.

Rinsho Byori. the Japanese Journal of Clinical Pathology

|June 25, 2002

Summary

This summary is machine-generated.

Gram staining remains a valuable diagnostic tool for rapid infection treatment and economic benefits. This review highlights its continued utility and introduces the Bartholomew & Mittwer staining method.

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Area of Science:

Microbiology
Clinical Diagnostics
Infectious Diseases

Context:

The Gram stain is a foundational microbiological technique for identifying bacterial pathogens.
Recent advancements in molecular and immunological methods have led to a decline in Gram stain utilization.
There is a need to re-evaluate the practical advantages of Gram staining in clinical settings.

Purpose:

To underscore the enduring significance of Gram staining in clinical microbiology.
To advocate for the continued use of Gram staining for rapid diagnosis and treatment initiation.
To introduce and explain the Bartholomew & Mittwer (B&M) staining protocol as an alternative to the Hucker method.

Summary:

This article reaffirms the diagnostic value of Gram staining, emphasizing its speed, cost-effectiveness, and crucial role in guiding initial antimicrobial therapy.
It details the Bartholomew & Mittwer (B&M) staining procedure, presenting it as a viable and potentially improved alternative to the traditional Hucker method.
The discussion highlights how Gram staining facilitates timely clinical decisions, contributing to better patient outcomes and efficient healthcare resource allocation.

Impact:

Reinforces the importance of Gram staining as a rapid, economical diagnostic method in clinical microbiology.
Promotes the adoption of the Bartholomew & Mittwer (B&M) method for improved Gram staining procedures.
Supports evidence-based clinical decision-making by emphasizing the utility of Gram staining in the early management of infections.