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[Antibody-based diagnostic assays with recombinant virus-like particles]
1Department of Virology II, National Institute of Infectious Diseases.
Nihon Rinsho. Japanese Journal of Clinical Medicine
|June 25, 2002
Summary
Researchers created virus-like particles for Norwalk-like viruses (NLVs) to detect antibodies. High antibody prevalence to 11 NLVs was found in healthy adults in Japan, with minimal cross-reactivity between genogroups.
Area of Science:
- Virology
- Immunology
- Gastroenterology
Context:
- Norwalk-like viruses (NLVs) are a significant cause of acute gastroenteritis globally.
- Previous diagnostic methods for NLVs have limitations in detecting diverse genotypes.
Purpose:
- To develop and validate virus-like particles (VLPs) for genogroup I and II NLVs using a baculovirus expression system.
- To assess the specificity and cross-reactivity of anti-NLV antibodies using enzyme-linked immunosorbent assays (ELISA).
Summary:
- VLPs representing 4 genogroup I and 7 genogroup II NLVs were successfully produced.
- ELISA using hyperimmune sera showed minimal cross-reactivity between genogroup I and II NLVs.
- A high prevalence of antibodies to 11 NLVs was detected in healthy adults in Japan.
Impact:
- The developed VLPs offer a promising tool for serological surveys and diagnostics of NLV infections.
- Findings suggest diverse NLV genotypes may contribute to oyster-associated gastroenteritis, while hospital outbreaks involve single genotypes.
- The study highlights the widespread exposure to NLVs in the Japanese adult population.