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[Importance of respiratory route in experimental salmonellosis in Balb/c mice]
Abstract:
Infection of Mice with S. typhimurium, was conducted by respiratory, oral and peritoneal routes. The results obtained showed that infection occurred more quickly by i. p. than by other routes. However i. n. inoculation is the more efficient concerning Bacteria concentration per Mousse to obtain the DL 100. A study of Salmonella growth in vivo was conducted in lungs, spleen, liver and blood. The results are discussed in relation to the calf respiratory Salmonellosis actually observed.
Insights
Respiratory inoculation of mice with Salmonella typhimurium (S. typhimurium) is most efficient for achieving lethal bacterial concentrations. Intraperitoneal injection leads to faster infection, while intranasal delivery optimizes bacterial load for Lethal Dose 100 studies.
Area of Science:
- Veterinary Microbiology
- Infectious Diseases
- Animal Models
Context:
- Salmonella typhimurium (S. typhimurium) infections pose a significant threat to animal health.
- Understanding infection routes is crucial for developing effective disease control strategies.
- Calf respiratory salmonellosis is a relevant clinical concern.
Purpose:
- To compare the efficiency of different inoculation routes (respiratory, oral, peritoneal) for S. typhimurium infection in mice.
- To determine the optimal route for achieving a high bacterial concentration per mouse to establish the Lethal Dose 100 (LD100).
- To investigate the in vivo growth of S. typhimurium in key organs and blood.
Summary:
- Infection occurred most rapidly via intraperitoneal (i.p.) injection.
- Intranasal (i.n.) inoculation proved most efficient for achieving the bacterial concentration required for LD100 determination.
- Salmonella growth was studied in the lungs, spleen, liver, and blood of infected mice.
- Findings are discussed in the context of naturally occurring calf respiratory salmonellosis.
Impact:
- Provides critical data for selecting appropriate animal models and inoculation methods in Salmonella research.
- Informs strategies for controlling S. typhimurium outbreaks, particularly respiratory forms.
- Contributes to understanding the pathogenesis of salmonellosis in a relevant mammalian model.