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[Expression of type I collagen and its receptor system in osteoblasts]
1Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu, Sichuan, P. R. China 610041.
Objective:
To study the expression of type I collagen and its receptor system-integrin alpha 2 beta 1 in different passages of osteoblasts.
Methods:
The expression of type I collagen and integrin alpha 2 beta 1 in the primary, sixth and fifteenth passage of osteoblasts were detected by S-P immunohistological staining technique, and their mRNA expression by quantity RT-PCR technique.
Results:
Type I collagen and integrin alpha 2 beta 1 were expressed in different passages of osteoblasts and there was no significant difference among three passages by immunohistological technique. Their mRNA expression was gradually decreased with subculture.
Conclusion:
Type I collagen promotes the adhesion and phenotype expression of osteoblasts through its receptor-integrin alpha 2 beta 1. The reductive expression of type I collagen-receptor system will decline the phenotype of osteoblasts.