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Related Experiment Videos

Developmental changes in ketogenic enzyme gene expression during sheep rumen development.

M A Lane1, R L Baldwin, B W Jesse

  • 1Department of Animal Sciences, Rutgers--The State University of New Jersey, New Brunswick 08903, USA.

Journal of Animal Science
|June 25, 2002
PubMed
Summary
This summary is machine-generated.

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Ketogenesis in lamb ruminal epithelium develops with age, independent of diet. Key enzyme gene expression, particularly 3-hydroxy-3-methylglutaryl-CoA synthase, increases by 42 days, matching beta-hydroxybutyrate production.

Area of Science:

  • Animal Physiology
  • Biochemistry
  • Ruminant Nutrition

Background:

  • Ketogenesis converts acetyl-CoA to ketone bodies (acetoacetate, beta-hydroxybutyrate/BHBA).
  • Hepatic ketogenesis occurs during fasting; ruminal epithelium produces ketones in mature ruminants from butyrate.
  • Understanding ruminal ketogenic enzyme gene expression is crucial for ruminant development.

Purpose of the Study:

  • To investigate ontogenic and dietary influences on ketogenic enzyme gene expression in developing lamb ruminal epithelium.
  • To correlate gene expression patterns with beta-hydroxybutyrate (BHBA) production capacity.

Main Methods:

  • Studied gene expression of acetoacetyl-CoA thiolase and 3-hydroxy-3-methylglutaryl-CoA synthase in ruminal epithelium from lambs aged 1-84 days.
  • Compared conventionally reared, milk-fed, and weaned/VFA-gavaged lambs.

Related Experiment Videos

  • Analyzed total RNA for mRNA concentrations.

    • Main Results:

    • Acetoacetyl-CoA thiolase and 3-hydroxy-3-methylglutaryl-CoA synthase mRNA levels increased with age, irrespective of diet.
    • 3-Hydroxy-3-methylglutaryl-CoA synthase mRNA showed a significant rise by 42 days of age in milk-fed lambs.
    • By 84 days, no dietary differences in gene expression were observed; patterns mirrored BHBA production rates.

    Conclusions:

    • Ketogenic capacity in lamb ruminal epithelium develops progressively with age.
    • The expression of ketogenic enzyme genes in ruminal epithelium is primarily age-dependent, not influenced by diet or volatile fatty acids (VFAs).