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Related Experiment Videos

An automated two-dimensional optical force clamp for single molecule studies.

Matthew J Lang1, Charles L Asbury, Joshua W Shaevitz

  • 1Department of Biological Sciences, Stanford University, Stanford, California 94305-5020, USA.

Biophysical Journal
|June 25, 2002
PubMed
Summary

We developed an advanced optical trap instrument to precisely measure single motor protein movement. This tool allows for detailed studies of kinesin

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Biotechnology

Background:

  • Motor proteins are essential for intracellular transport.
  • Understanding motor protein mechanics is crucial for cell biology.
  • Previous methods had limitations in precisely controlling forces on single molecules.

Purpose of the Study:

  • To develop and validate a next-generation optical trapping instrument.
  • To enable precise force clamp measurements of single motor protein motility.
  • To investigate the effects of applied loads on kinesin movement.

Main Methods:

  • Construction of a computer-controlled optical trapping instrument.
  • Utilized acousto-optic deflectors and a 3D piezo stage for precise control.
  • Implemented an initialization sequence including optical force microscopy for accurate positioning.

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  • Operated the instrument in a two-dimensional force clamp mode.

    • Main Results:

    • Demonstrated the capability to apply fixed magnitude and direction loads to motor proteins.
    • Achieved high precision in bead height (+/-4 nm) and lateral position (+/-3 nm) adjustments.
    • Presented preliminary data on kinesin motor movement under various load conditions.
    • Validated the dynamic control of trap and stage for constant force application.

    Conclusions:

    • The developed optical trapping instrument offers enhanced flexibility and automation for studying motor protein dynamics.
    • The force clamp functionality allows for detailed investigation of motor protein mechanics under controlled loads.
    • Preliminary data showcase the instrument's potential for advancing our understanding of kinesin motility and force generation.