Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Anisotropic diffusion in mitral cell dendrites revealed by fluorescence correlation spectroscopy.

Arne Gennerich1, Detlev Schild

  • 1Physiologisches Institut, Universität Göttingen, D 37073 Göttingen, Germany.

Biophysical Journal
|June 25, 2002
PubMed
Summary

Fluorescence correlation spectroscopy revealed anisotropic diffusion of TMR-dextran in Xenopus laevis tadpole mitral cell dendrites. Intradendritic microtubules likely cause this restricted molecular movement.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond Binding Affinity: How a Conformation-Specific Salt Bridge Tunes KIF1A Mechanochemistry.

Cytoskeleton (Hoboken, N.J.)·2026
Same author

The P3018S disease variant reveals how dynein's trailing motor sets ensemble velocity.

bioRxiv : the preprint server for biology·2026
Same author

Pathogenic KIF1A R350 mutations disrupt a conserved and conformation-dependent kinesin-tubulin salt bridge.

Nature communications·2026
Same author

Publisher Correction: Adaptor-mediated recruitment of three dyneins to dynactin enhances force generation.

Nature cell biology·2026
Same author

Adaptor-mediated recruitment of three dyneins to dynactin enhances force generation.

Nature cell biology·2026
Same author

Pathogenic KIF1A R350 Variants Disrupt A Conserved Kinesin-Tubulin Salt Bridge.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Biophysics
  • Cell Biology
  • Neuroscience

Background:

  • Fluorescence correlation spectroscopy (FCS) measures single-molecule kinetics.
  • Understanding molecular diffusion within cellular structures is crucial for cell function.
  • The plasma membrane acts as a boundary influencing diffusion.

Purpose of the Study:

  • To investigate the diffusion dynamics of tetramethylrhodamine (TMR)-dextran in mitral cell dendrites.
  • To analyze the effect of cellular structures on molecular transport.
  • To establish accurate models for interpreting FCS data in complex cellular environments.

Main Methods:

  • Utilized FCS to measure the diffusion of 10 kDa TMR-dextran in cultured Xenopus laevis mitral cells.
  • Applied models of anisotropic diffusion to analyze data from dendrites.

Related Experiment Videos

  • Compared diffusion rates within dendrites and somata to diffusion in bulk water.

    • Main Results:

    • Diffusion of 10 kDa TMR-dextran in mitral cell dendrites exhibited anisotropy.
    • Axial diffusion along dendrites was slowed by 1.1-2.1 fold compared to water.
    • Lateral diffusion was significantly restricted (10-100 times slower than in water).
    • Diffusion in somata was isotropic and 1.2-2.6 times slower than in water.

    Conclusions:

    • Anisotropic diffusion in dendrites is best explained by a model accounting for the plasma membrane boundary.
    • The dense, parallel microtubule network within dendrites is a potential cause for the observed diffusion anisotropy.
    • FCS measurements require consideration of cellular architecture for accurate interpretation of molecular diffusion.