Related Experiment Videos
[The study of new bio-membrane-bred epidermal cell grafts]
1Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, P. R. China 200443. jscao@ecmu.org.cn
Objective:
The human epidermal cells were bred on a kind of bio-membrane, the bio-brane, in engineering a kind of new epidermal substitute, the bio-membrane bred cell graft.
Methods:
Fresh and frozen grafts of biomembrane bred epidermal cells were transplanted into the full-thickness wounds of nude mice and those received simple Bio-brane were served as control. The wounds of the two groups were observed daily and biopsy was taken on the 3, 5, 7, 10, 21 and 35 days respectively.
Results:
Epidermal cells could be cultured in vitro on the bio-membrane reaching the sub-saturated state of 60 to 70 percents. The bio-membrane after being grafted the epidermal cells continued to proliferate and differentiate to form a layer of new epidermis. There was no difference between the fresh and the frozen bio-membranes.
Conclusion:
Bio-membrane bred with epidermal cells could be a kind of ideal epidermal substitute.