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[The separation and detection of the bioactive proteins in nerve regeneration conditioned fluids]
1Department of Plastic Surgery, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200011.
Objective:
To understand the biological activities of the nerve regeneration conditioned fluid (NRCF).
Methods:
Nerve regeneration chamber was made by using silicone tube bridging distal and proximal ends of severed SD rat's sciatic nerve. The biological activities of the proteins in NRCF, which were separated by natural polyacrylamide gel electrophoresis (PAGE), were analysed by being cocultured with excised neonatal dorsal root ganglia (DRG).
Results:
Eight separated protein bands of NRCF were observed between 67-669 ku in molecular weight, and the protein bands between 232-440 ku showed strong neurotrophic and chemotactic function.
Conclusion:
NRCF has the promoting effects on nerve regeneration.