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[Immunohistochemical study of S-100 protein in degenerative nerve after different pathological brachial plexus
1Department of Hand Surgery, Huashan Hospital, Shanghai Medical University, Shanghai, P. R. China 200040. shaonanhu@hotmail.com
Objective:
To explore the regularity of the change of S-100 protein in degenerative nerve after different pathological brachial plexus injuries.
Methods:
Eighty SD rats were randomly divided into two groups, right C5, C6 preganglionic injury, and postganglionic injury. The distribution and content of S-100 protein in distal degenerative nerve were detected after 1, 2, 3 and 6 months of injury by immunohistochemical methods.
Results:
The S-100 protein was mainly distributed along the axons. The S-100 protein positive axons of each time interval decreased after operation, with significant difference from normal nerves (P < 0.01). There was no statistically significant difference among 1, 2, 3 and 6 months group (P > 0.05). The S-100 protein stain of postganglionic group was negative.
Conclusion:
In preganglionic injury, the functional expression of Schwann's cells in the distal stump keeps at a certain level and for a certain period. Since Schwann's cell has inductive effect on nerve regeneration, it suggests that the distal nerve stump in preganglionic injury can be used as nerve grafts.