Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

6.6K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
6.6K
Reproductive Cloning01:27

Reproductive Cloning

32.3K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
32.3K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.2K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.2K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.0K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.0K
Cell Lines01:16

Cell Lines

9.8K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
9.8K
CRISPR01:59

CRISPR

57.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular signature of human endometrial stem/progenitor cells at the single-cell level.

Stem cell reports·2026
Same author

Chromosome X dosage modulates development of aneuploidy in genetically diverse mouse embryonic stem cells.

Stem cell reports·2026
Same author

Cleavage stage versus blastocyst stage transfers in patients with a single zygote: an emulated target trial.

Human reproduction (Oxford, England)·2026
Same author

Cell therapy for age-related macular degeneration.

Mammalian genome : official journal of the International Mammalian Genome Society·2026
Same author

Improving rigor and reproducibility through implementation of the ISSCR standards for human stem cell use in research.

Stem cell reports·2026
Same author

CAR-NK Engineering to Overcome TME Barriers.

Cells·2026
Same journal

Investigating the effect of preconception untreated subclinical hypothyroidism (TSH above normal range and below 6.0 mIU/L) on assisted reproductive technology (ART) outcomes.

Human fertility (Cambridge, England)·2026
Same journal

Does ICSI timing impact cumulative live birth and perinatal outcomes - a cohort study.

Human fertility (Cambridge, England)·2026
Same journal

Association between dietary quality indices and female infertility: a population-based study using NHANES 2013-2020.

Human fertility (Cambridge, England)·2026
Same journal

Contact experiences among donor conception parties: donor conceived persons, donors, parents and same-donor siblings - a mixed methods systematic review.

Human fertility (Cambridge, England)·2026
Same journal

Single mothers by choice in Hungary: motivations for solo motherhood and the importance of genetic ties.

Human fertility (Cambridge, England)·2026
Same journal

Donor conception and family connection: beyond genetic reductionism in the postgenomic era.

Human fertility (Cambridge, England)·2026
See all related articles

Related Experiment Video

Updated: Dec 19, 2025

Author Spotlight: Exploring Cloning Techniques for Full-Length DNA Fragments
04:18

Author Spotlight: Exploring Cloning Techniques for Full-Length DNA Fragments

Published on: May 17, 2024

962

Human cloning 2001.

David L Healy1, Gareth Weston, Martin F Pera

  • 1Department of Obstetrics and Gynaecology, Monash University, Melbourne, Victoria, Australia.

Human Fertility (Cambridge, England)
|June 26, 2002
PubMed
Summary
This summary is machine-generated.

Human cloning, including natural twins and fertility treatments, is reviewed. Reproductive cloning, unlike therapeutic cloning, lacks a safe animal model, making

More Related Videos

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
06:15

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Published on: July 6, 2022

2.5K
Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

8.5K

Related Experiment Videos

Last Updated: Dec 19, 2025

Author Spotlight: Exploring Cloning Techniques for Full-Length DNA Fragments
04:18

Author Spotlight: Exploring Cloning Techniques for Full-Length DNA Fragments

Published on: May 17, 2024

962
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
06:15

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Published on: July 6, 2022

2.5K
Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

8.5K

Area of Science:

  • Reproductive medicine and developmental biology.
  • Bioethics and clinical applications of cloning technologies.

Background:

  • Human cloning is examined from a clinical viewpoint.
  • Monozygotic twins represent natural human clones, with increasing incidence.
  • Assisted reproductive technologies have produced iatrogenic human clones for decades.

Purpose of the Study:

  • To provide a clinical perspective on human cloning.
  • To differentiate between therapeutic and reproductive cloning.
  • To assess the safety and ethical considerations of human cloning.

Main Methods:

  • Review of existing literature on human cloning.
  • Clinical analysis of natural and iatrogenic human cloning.
  • Ethical and safety evaluation of reproductive cloning techniques.

Main Results:

  • Natural and iatrogenic human cloning are established phenomena.
  • A critical distinction exists between therapeutic and reproductive cloning.
  • No safe and dependable animal model currently exists for human reproductive cloning.

Conclusions:

  • Reproductive cloning in humans is medically unacceptable without a validated safe animal model.
  • Therapeutic cloning using embryonic stem cells requires careful ethical and scientific consideration.
  • Further research into safe and reliable cloning models is essential before human reproductive cloning can be considered.